The Texas Department of Agriculture has enacted a second quarantine in the Rio Grande Valley after citrus greening was found in a residential grapefruit tree in Mission.

The restrictions encompass a 5-mile radius of the positive find and restrict the movement of citrus nursery trees, according to a news release.

Growers within the zone who want to harvest fruit for transport to an outside packinghouse must first treat trees with an approved insecticide and then harvest within seven days of application.

Harvest crews also must work to remove leaves, stems and other plant material before moving the bins outside the quarantine area.

The Mission discovery is the second time a greening-infected tree has been found in the Texas citrus-producing region. In January 2012, the state and U.S. Department of Agriculture confirmed greening in a commercial orange grove near San Juan.

The discoveries come on top of numerous psyllids from the Rio Grande Valley that have been tested and found positive for citrus greening, also known as huanglongbing.

Although the positive psyllids don’t result in regulatory action, they are a good indication that the disease is present in the area, according to the website.